Carlos Elena-Real

2 Questions 6 Answers 0 Followers

Questions related from Carlos Elena-Real

How to analyse a CD spectra of proteins to get secondary elements proportion?

I have been measuring circular dichroism spectra between 250 and 190 nm for proteins. I would like to calculate the percentage of each secondary structure element . Does anyone know any software...

03 March 2017 6,703 3 View

How to measure small peptide concentration?

I want to measure the small peptide (2-3 kDa, 25 residues) concentration, but it hasn´t any aromatic residue, so it is not possible to use 280 nm absorbance. Moreover, Bradford assay did not work,...

04 April 2016 431 17 View