What is the best method to calculate protein thermostabilty by using computational analysis

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Jennifer Wells

I don't know any software but you could just do isothermal titration calorimetry?

Guillaume Blanchet

Hi,

I don't know either... And I will be surprised if such a computation is accurate and reliable.

You can also obtain your protein thermostability using the DSF (fluorescent Thermal Stability) -see paper attached-. That can be easily done with a PCR machine and a plate reader if you have the protein sample of course.

Best wishes,

Guillaume

Carlos Elena-Real

Experimentally, you can use Differential Scanning Calorimetry (DSC) . Its very useful for thermal stability.