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Questions related from Janith Wanigasekara
I need to fix the 3D tumor spheroid that has been constructed using the low attachment plate method for confocal imaging. We are having problems with fixation without changing the sphere shape....
10 May 2021 7,444 4 View
Can anyone recommend me a method or software to quantify the average amount of PI staining in tumorsphere relative to the size of the tumorsphere by using confocal images?
13 November 2019 4,263 1 View
Do I need to convert 3D tumorsphere into single cell suspension before the alamarBlue treatment in assay? Or else can I use the intact tumorsphere directly for the alamarBlue treatment? Are there...
07 September 2019 9,062 4 View
What is the best method to calculate lattice constant using X-ray diffraction pattern
14 September 2018 8,663 3 View
I need to compare isolated and over expressed alkaline protease thermo stability with already industrially applied alkaline protease.What is the best software for that thermostability calculation...
12 July 2017 7,607 3 View
I have to analyze Zn,Se,Cu,Cr Fe,Cd, As, Pb, F and Co by using ICP-MS method. Please give an idea about the internal standards i suppose to use.
30 June 2017 9,862 5 View
I am trying to find out whether the Michels solution used in histopathology analysis could be used and stored in room temperature. Or what would be the optimal temperature to store this solution.
09 May 2017 854 3 View
I am looking for the best blood collection tube for sample collection for the trace metal analysis with ICP -MS. Also can anyone suggest what segment of the blood is more suitable for the trace...
09 May 2017 2,761 4 View
I used RT-PCR to determine brain derived neurotrophic factor gene expression and measure amount of protein in rodent brain by using western blot. What is the most suitable method to determine...
29 April 2017 7,019 4 View
