What would be the approach to remove repetitive and non-unique sequence motifs/reads with low complexity and increase sequencing capacity with unique genome elements, suitable for "unknown" pathogen detection?
Cot denaturation/renaturation?
Dinesh Kumar Sahu
Normalization by DSN enzyme is a best way to remove the repetitive DNA.
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Ivan Brukner
thx Dinesh
can you suggest full protocol for DSN enzyme utility and some examples of reduction in rep. elements?
Please send to [email protected]
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Oadi Matny
There are many kits is used to clean pcr product before go to sequencing technique, you can fiend it out in online.
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