Ivan Brukner

7 Questions 16 Answers 0 Followers

Questions related from Ivan Brukner

Clinical research and ethics?

Is this correct? If your cerebrospinal fluid is positive (Bacteremia), only one-two (1-2

30 October 2018 5,741 0 View

Did you decide about amplifiction? Taq-based...Phi29...?

Taq-like enzymes (for "short" ) fragments Phi29-for long DNA (10 000+Kb) Is rare event "selected" before or after amplification? how many rare events you expect per sample to analyse? all the...

04 April 2017 3,267 0 View

How to eliminate circular mitochondrial DNA before Phi29 driven WGA of human tissue?

Phi 29 has a rolling amplification capacity and mtDNA is over-amplified (or any circular DNA) versus rest of samples. Phi29 prefer's long template (if linear) versus short (linear) one. If anyone...

05 March 2016 9,769 1 View

What is the capacity of current MALDI TOF microorganism database, applicable to clinical diagnostics?

Bacterial and fungal identification capacity in clinical diagnostics, under assumption that blood culture is positive (in 60% cases of sepsis that is the case). Can it detect all? 500? 1000?...

13 November 2015 6,182 1 View

Does CFX Bio-Rad 96 qPCR have to have ROX normalisation for monoplex reaction?

is there fundamental need for CFX-96 well Bio-Rad assay to use Rox day for qualitative assay? thanks in advance

06 July 2015 6,726 4 View

What is the number of single copy versus repetitive sequence reads of human genomic DNA ?

Any idea how much DNA reads will correspond to Alu, Line, Sine...using NGS, considering capacity of 1000000 reads with 150nt length on average?

20 August 2014 1,183 2 View

What is the best method for removing repetitive DNA before sequencing?

What would be the approach to remove repetitive and non-unique sequence motifs/reads with low complexity and increase sequencing capacity with unique genome elements, suitable for "unknown"...

06 August 2014 3,263 3 View