Ivan Brukner

2 Questions 8 Answers 0 Followers

Questions related from Ivan Brukner

How to eliminate circular mitochondrial DNA before Phi29 driven WGA of human tissue?

Phi 29 has a rolling amplification capacity and mtDNA is over-amplified (or any circular DNA) versus rest of samples. Phi29 prefer's long template (if linear) versus short (linear) one. If anyone...

05 March 2016 9,725 1 View

Does CFX Bio-Rad 96 qPCR have to have ROX normalisation for monoplex reaction?

is there fundamental need for CFX-96 well Bio-Rad assay to use Rox day for qualitative assay? thanks in advance

06 July 2015 6,673 4 View