What is the best method for preparation of probiotic feed (L. acidophilus 109 CFU/g) on broiler chickens?

23 July 2016 9 4K Report

I have done the isolation of lactic acid bacteria and also studied probiotic potential studies. I have selected Lactobacillus acidophilus and wanted to study its effect as probiotic in invivo trials (i.e. on broiler chickens).

My question is how to prepare probiotic L. acidophilus 109 CFU/g for the poultry feeding. In what procedure the cell of L. acidophilus should be mix with poultry feed to obtain 109 CFU/g.

Should I Lypholize the cells or just mix the dry cells to feed??

C.A. (Kees) Kan

Several points of attention.

1; broilers grow better on pelleted than on meal feed, but probiotics may not survive pelleting

2. what is the difference between lyophylized and dry cells? Both are dry in my opinion but the moisture content might differ (slightly)

3. To obtain an even distribution of feed additives, use a premix of around 1 % of the prepared, containing the concentrated form of additive (so at least 10e11 cfu/g)

Biswaranjan Pradhan

Hi Anila,

Lactobacillus acidophilus is not a spore forming bacteria. It is easier for spore forming probiotic bacteria to be pelleted and mixed with food in appropriate concentration without affecting their viability. But in your case probiotics administration with food (With appropriate dose) may not be a good idea. I would suggest you to orally gavage the probiotic with saline. In this way you can have better control over the probiotics dose and viability.

Anila Kumari

thank you C.A. (Kees) Kan and Biswaranjan Pradhan for your suggestions.

Bhoomi Patel

Hi Anila,

If you prepare the dry biomass of your organism and than that dry powder of organism mix with food and than given the dosage of organism to chicken.

Javad Ranjbari

I am working on commercial probiotic. I want to know how can I count the number of bacteria in 1 gr of probiotics(CFU/gr)?

C.A. (Kees) Kan

Oral gavage is not animal friendly and impossible to carry out on large numbers of young chicks. So, not a way to test a probiotic in broilers.

Bijaya K. Swain

I agree with C.A. Kan that it is not possible to carry out oral gavage in broiler experiment due to large sample size.

Shakira Ghazanfar

I am also agree with CA Khan....we must not use oral Gavage in broiler

Chen Yu

Dear esteemed colleague,

I hope this message finds you well and thriving in your research endeavors. Your inquiry regarding the optimal method for the preparation of probiotic feed, specifically incorporating Lactobacillus acidophilus at a concentration of 10^9 CFU/g, for broiler chickens is both timely and significant. The incorporation of probiotics into poultry feed has been recognized for its potential to improve gut health, enhance immune response, and increase growth performance in broiler chickens. Herein, I provide a comprehensive and logical approach to formulating probiotic feed effectively.

Selection of Probiotic Strain: The initial step involves the selection of a high-quality, poultry-specific strain of L. acidophilus. It is imperative to source the probiotic from a reputable supplier to ensure its viability, purity, and concentration. The chosen strain should have documented evidence of stability and efficacy in poultry.

Probiotic Preparation: Prior to incorporation into the feed, the probiotic culture must be propagated under optimal conditions to achieve the desired concentration of 10^9 CFU/g. This necessitates a sterile, controlled fermentation process, followed by careful drying to preserve the viability of the probiotics. Freeze-drying or spray-drying are commonly employed methods, with the former being preferred for its ability to maintain higher levels of live bacteria.

Feed Formulation: The next step involves the homogeneous mixing of the dried probiotic preparation with the broiler feed. It is crucial to ensure that the mixing process does not compromise the viability of the probiotics. Employing a gentle, thorough mixing procedure can help achieve a uniform distribution of the probiotic throughout the feed.

Stability and Storage: Probiotic feed must be stored in conditions that preserve the viability of the L. acidophilus. This includes protection from moisture, heat, and direct sunlight. The feed should be kept in a cool, dry place, and ideally used within a short period after preparation to prevent a significant decline in probiotic concentration.

Dosage and Administration: The effective dose of L. acidophilus must be maintained at 10^9 CFU/g of feed to ensure the health benefits are realized. Continuous administration of the probiotic feed throughout the broiler's growth period is recommended for optimal results.

Monitoring and Evaluation: It is essential to regularly monitor the health status and growth performance of the broiler chickens receiving the probiotic feed. This includes assessing gut health, feed conversion ratio, and overall morbidity and mortality rates. Adjustments to the probiotic feed formulation may be necessary based on these observations to optimize its efficacy.

Regulatory Compliance: Ensure that the use of L. acidophilus as a feed additive complies with local and international regulations governing the use of probiotics in animal feed. This includes adherence to guidelines regarding the safety, labeling, and marketing of probiotic products.

In conclusion, the preparation of probiotic feed containing L. acidophilus at a concentration of 10^9 CFU/g for broiler chickens involves a systematic approach that prioritizes the selection of a suitable strain, careful preparation and formulation of the feed, and diligent monitoring of its effects on poultry health. By adhering to these guidelines, you can maximize the benefits of probiotic supplementation in broiler production.

Should you require further assistance or have additional queries, please do not hesitate to reach out.

Warm regards,

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