Anyone can help please about the oxygen level of fermentation of actinobacteria in the bioreactor?

I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance

02 March 2018 5,652 8 View

What is the best solvent used for antimicrobial activity screening other than acetone and ethyl acetate?

08 September 2017 7,098 4 View

No title

08 September 2017 5,381 12 View

Can soil actinomycetes isolates tolerate high temperatures like 42?

What's the highest temperature could actinomycetes isolates tolerate and what's maximum time for these high temperatures Please anyone help

06 July 2016 9,097 4 View

What is the optimum concentration of Genomic DNA and primers to get perfect PCR products?

I want to get sharp bands of PCR products on the gel electrophoresis without any tailings, primer dimers or non-specific products. What is the best concentration of both primers and DNA to get...

11 December 2015 9,610 7 View

What is the best antifungal used in isolation of a mix of bacteria and fungi?

I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...

11 December 2014 1,483 10 View

What is the best method for identification of actinomycetes biochemically?

I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards

09 October 2014 9,496 3 View

Can anyone help me to collect non-clinical microbial strains producing biologically active secondary metabolites?

If I want to have bacterial samples which can produce biologically active secondary metabolites, what is the best way to do that and what are the sources from which I can collect them? Please help...

09 October 2014 8,939 16 View

Does media selection affects the production of novel biologically active compounds from the microbes?

09 October 2014 696 7 View

What is the best media for cultivation of actinomycetes to assist production of biologically active secondary metabolites?

09 October 2014 6,071 9 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View