If I want to make glycerol broth for my isolates, what is the best method do that and what are the best proportions of the glycerol and the broth? Please provide a detailed protocol, thanks.
Please help me if you can
I'm asking about the optimum oxygenation level or range for bioreactor fermentation of some soil actinobacteria for production of natural products. Thanks in advance
02 March 2018 5,775 8 View
I have problem that ethyl acetate and acetone have some antimicrobial activity against some of my standards strains, so I shouldn’t use them for antimicrobial activity not to affect my results...
08 September 2017 7,194 4 View
I’d like to know how to prepare the standards strains broth in the best way and how to do the test and interpreter the results I know that it should be done in triplicates but I’m not sure about...
08 September 2017 5,479 12 View
What's the highest temperature could actinomycetes isolates tolerate and what's maximum time for these high temperatures Please anyone help
06 July 2016 9,203 4 View
I want to get sharp bands of PCR products on the gel electrophoresis without any tailings, primer dimers or non-specific products. What is the best concentration of both primers and DNA to get...
11 December 2015 9,748 7 View
I have some environmental samples that contain both fungi and bacteria and I want to isolate only bacteria What is the best way to do that and if I want to add antifungal in the media, what is the...
11 December 2014 1,584 10 View
I had isolated some isolates suspected to be actinomycetes. What is the best method for identification of them biochemically? Please help if you can Best regards
09 October 2014 9,601 3 View
If I want to have bacterial samples which can produce biologically active secondary metabolites, what is the best way to do that and what are the sources from which I can collect them? Please help...
09 October 2014 9,052 16 View
If I want to have bacterial samples producing novel biologically active secondary metabolites, what is the best way for selection of the media for cultivation of these bacteria to produce new...
09 October 2014 805 7 View
If I want to obtain isolates of actinomycetes which produce novel biologically active secondary metabolites, what is the best media for cultivation of these actinomycetes to help them produce...
09 October 2014 6,463 9 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View