Hi, is is not sufficiently clear for me, for what special purpose; this is always important to know: Do you already have a keratinase enzyme and look for the best method for the determination of enzyme activity, kinetics and other parameters, or:

Do you wish to enrich and isolate microorganisms (bacteria and/or fungi) which posses a keratinase which you wish to characterize?

Please, specify your needs.

First of all thanks to your consideration, Yes sir, I want to determine or optimization of keratinase production and enzyme activity from Keratin (Chicken feathers) by keratinophilic fungi and keratinophilic/keratinolytic bacteria in liquid media. So, What is the best method for degradation of feathers in liquid ,media for keratinase enzyme production and keratinase enzyme activity by fungi/bacteria?

Well, many researchers worked on this topic, and I will give you an old, but good reference:

http://www.ncbi.nlm.nih.gov/m/pubmed/7487006/?i=2&from=muller%20r,%20feather

For newer papers on this, search PubMed or other databases by using search terms such as feather, keratin, biodegradation.

Good luck, Tarun!

Yes Sir, Thanks a lot for your kind support. thanks again.

Sorry, I am a female person; also female scientists do exist.

Please find the papers for your reference

Best luck

https://www.researchgate.net/publication/317500618_Characterization_of_keratin_microparticles_from_feather_biomass_with_potent_antioxidant_and_anticancer_activities

https://www.researchgate.net/publication/317328078_Statistical_investigation_of_extraction_parameters_of_keratin_from_chicken_feather_using_Design-Expert

https://www.researchgate.net/publication/316467223_Dissolution_and_characterization_of_biofunctional_keratin_particles_extracted_from_chicken_feathers

https://www.researchgate.net/publication/301810903_Sustainable_Management_of_Keratin_Waste_Biomass_Applications_and_Future_Perspectives

Article Characterization of keratin microparticles from feather biom...

Article Statistical investigation of extraction parameters of kerati...

Article Dissolution and characterization of biofunctional keratin pa...

Article Sustainable Management of Keratin Waste Biomass: Application...