Hello everyone,
I have started a master thesis and to search for previous work.
The definition is to collect manual explanation and judging automatically created explanations related to the manual explanation.
I am doing qRT-PCR to check the expression of certain miRNA and mRNA in the mitochondrial fraction. But I am not able to determine which reference gene I should be taking in the case of the...
19 June 2023 4,110 2 View
I have recently done RNA-IP assay by pull-down of my protein of interest and my further applications involve the analysis of bound RNA to it. For this, I did RNA-IP and isolated RNA from the...
10 May 2023 359 0 View
I am getting amplification and specific melt-curves in Non-Template Control sample (with water instead of cDNA). I ordered some primers and reconstituted them to 100 uM and diluted them to 10 uM...
07 April 2023 1,969 2 View
I have been trying to clone an shRNA for a gene knock-down in pLKO.1 vector but I am not able to get the clone. I don't know what the problem is. I have tried annealing the oligos at 95 C in a PCR...
29 September 2022 8,343 5 View
Hello, I need to plot Taylor diagrams for some of my analysis. However, I am not sure how can I plot them either in excel or Matlab. I have searched on several sites, I could not reach any final...
17 December 2020 5,599 3 View
I want to extract NDVI values on each pixel (like ASCII files in GIS) for every time step over the last 20 years period for given catchments using Google earth engine. I don't know as how to...
14 October 2018 9,382 6 View
I have got some governing equations related to beam vibration (Figure attached) . Such equations are easily solved as eigen value problem. Two of them are coupled. It has 3rd order terms also. I...
23 March 2018 1,256 6 View
I have some coupled nonlinear ordinary differential equations. 2 equations have two 2nd order derivative together. Can someone tell me how I can get the response, numerically? Ci =...
19 January 2018 941 11 View
“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...
11 August 2024 2,483 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...
10 August 2024 4,347 1 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View