I need to quantitatively measure cell lysis of bacteria that are already dead.
This is not my field, but I imagine you can either perform counts of cells (per given area) or mean/sd of the cells (within of a given area). It doesn't seem to be more complicated than that, unless I am missing something?
Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...
17 February 2021 2,383 3 View
Can anyone answer the question: Are there possibilities to paleontologists to use nanotechnology for solving problems related to different areas of their research?
06 December 2020 5,335 3 View
I wonder if anyone can thankfully provide me with a free software to analyze data mining and/or artificial intelligence.
16 November 2020 9,723 13 View
Current I am using calcium hydroxide but at higher pH it make gel type material
09 November 2020 3,321 3 View
I couldn't find it in any books and articles. I want to series feed two-slot antenna arrays but it is not giving me enough bandwidth. I want to design an equivalent circuit for the series feed...
19 October 2020 4,664 3 View
Complement of the day, Kindly help me to understand poses per run and its importance to increase the activity of compound or decrease it. my greetings,
28 September 2020 9,194 2 View
Added Si to nutrient solution carrying metals and mineral nutrients under acidic conditions, therefore, it's necessary that they provide evidence that Si addition does not cause pH fluctuations...
26 September 2020 7,706 2 View
20 September 2020 3,125 3 View
Some types of plant herbs were extracted with alcohol in order to obtain their pigments and add specific chemicals materials to obtain their nano scale features ... The question is, are they...
30 August 2020 4,718 4 View
I have generated a .str file from .pdb file using CGenFF server. But whenever I try to convert the .str file to gromacs specific topology using: python3 cgenff_charmm2gmx_py3_nx2.py JZ4...
24 August 2020 3,593 3 View
02 March 2021 3,060 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View