I need to quantitatively measure cell lysis of bacteria that are already dead.
This is not my field, but I imagine you can either perform counts of cells (per given area) or mean/sd of the cells (within of a given area). It doesn't seem to be more complicated than that, unless I am missing something?
I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...
31 July 2024 9,757 3 View
After checking the eligibility criteria, are both high-quality and low-quality articles included in the review? What is Cochrane's recommendation?
02 July 2024 5,322 4 View
So I have a structure with a pile foundation and a gravity load acting on the structure. The demand curve is drawn by running an elastic analysis. Pile-Soil is modelled as p-y, t-z, and q-z...
14 June 2024 5,220 0 View
PPPPlease
27 May 2024 8,219 1 View
I have read somewhere that absorbance without stop solution is about half those with stop solution. Is this true?
14 March 2024 5,575 3 View
Please, I urgently need to remove and delete this article preprint "Cytotoxicity and genotoxicity reveal the link between acute cadmium exposure and Alzheimer’s disease" from Researchgate. I can't...
13 March 2024 8,002 2 View
I am working on Battery Pack Thermal Analysis for an Electric Vehicle. Whenever I get to the point of pressing "Run Calculation", the solver displays 1 iteration only then displays some info and...
11 February 2024 3,981 1 View
Similar to what we typically do with CCD, but in a randomized manner!
04 February 2024 3,597 2 View
How do we change the fluorescence intensity of the confocal microscopy to quantitative result by image J?
15 January 2024 2,954 3 View
Hello, I have performed a simulation of Sodium Montmorillonite swelling due to water adsorption. However, I am not sure if the simulation is correct. I followed the following steps: 1. A model of...
17 December 2023 10,018 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View