What is the best kit to extract DNA from oral swabs ?

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I need book take about role theory?

please tell me how i can have book take about role theory

30 May 2024 7,042 0 View

Calprotectin in mice feces?

Hi, Did anyone measure calprotectin in mice feces using ELISA? if so, did you dilute the feces supernatant?

25 February 2024 2,058 0 View

What are the recent most important topics for research now adays?

Suggest for me topics I can write about

19 October 2023 9,191 4 View

Looking for an opportunity with dermatological cases writing or editing to utilize and improve research skills and knowledge. ?

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14 October 2023 6,462 2 View

I want to do sulforhodamine B (SRB) assay, in which i would like to add the treatment for 6 days, whats the best practice?

I usually treat the cells for maximum 3 days, what i usually do is that i seed for 24 hrs (180uL cell suspension in each well (96-well plate) and then on the next day i add the treatment on top of...

06 October 2023 5,719 0 View

New trends in liver transplantation?

post liver transplantation care , health outcomes

07 June 2023 4,382 0 View

What we shoud write on Literature Review?

I want deep understand how to write Literature Review as professional research

04 May 2023 6,639 4 View

Why do we use catalase to inhibit hydrogen peroxide H2O2 in lactic acid bacteria ?

if the LAB incubated anaerobically , why do we use catalase to inhibit H2O2 ? hydrogen peroxide produced when the oxygen is available so why do we need to inhibit it in the absence of oxygen ?

27 February 2023 1,497 1 View

Distinguishing the antimicrobial effect of bacteriocin from other antimicrobial compounds?

Hello. We are working on LAB bacteriocins to detect the antimicrobial activities against pathogenic bacteria. We grew LAB on MRS broth and obtained CFS, it showed inhibition zone , but what is the...

12 February 2023 4,363 3 View

How to measure the chemical oxygen demand (COD) of cooking oil?

I need to measure the chemical oxygen demand for cooking oil that I am using for Anaerobic digestion. I tried high range, high range plus, and low ranges COD kits, and still no luck. After...

08 October 2022 1,730 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View