What is the best kit/reagent to use for extracting total RNA and miRNA from human adipose tissue?

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How do I prepare an RNA gel?

I am looking to assess the quality of some RNA that I have extracted from human adipose tissue. Currently using a page supported by ThermoFisher...

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How to design good primers?

Other than assessing for temperature of hairpin structure (for both forward and reverse primers), self-dimer Delta G, and hetero-dimer Delta G, is there anything else to look at before ordering...

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Are my adipose tissue samples still viable after being in a -80 that went down on us over the weekend?

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How do I design primers/probes for eukaryotes?

I am seeking assistance in regards to designing primer/probe sequences for eukaryotes (human). I usually go to NCI Gene --> enter the gene of interest --> click on FASTA sequence -->...

04 May 2018 5,062 2 View

What is the amount of RNA (adipose tissue origin) used for 1-step RT-qPCR?

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What is the best way to store protein extracted from adipose tissue samples after QIAzol homogenization?

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Issue extracting RNA from adipose tissue

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Which bacteria are suitable for quorum sensing screening?

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How to remove fatty material from plant extract?

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Can water be extracted from Olivine?

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Hendra Susanto

I think you can use Qiagen RNeasy lipid tissue kit to isolate total RNA from adipose tissue.

Mikey Smith

I am leaning towards that kit. The only thing that I am cautious about is the required purchase of additional materials to isolate miRNA. I am now looking Qiagen’s RNeasy Mini Kit as it allows for separate isolation of mRNAs and miRNAs. Only thing is, I don’t know how the kit does with lipid tissues

Josh Kellogg

I haven't done miRNA, but for mRNA from adipocytes, I used TRIzol from Life Technologies; it was a very easy work-up. If I can answer any other questions, let me know!

Article Alaskan seaweeds lower inflammation in RAW 264.7 macrophages...

Hello Josh! Thank you for your response. I was biased against using TRIzol but have since warmed up to the idea. What I am thinking is to use TRIzol to isolate the total RNA and proteins (ELISA is later down-stream analysis). After using TRIzol to extract total RNA, I would use the RNeasy Mini Elute Kit (Qiagen) to isolate mRNA and miRNA. In addition I would follow up with an on-column DNase digestion step.