Mikey Smith

8 Questions 6 Answers 0 Followers

Questions related from Mikey Smith

How to design good primers?

Other than assessing for temperature of hairpin structure (for both forward and reverse primers), self-dimer Delta G, and hetero-dimer Delta G, is there anything else to look at before ordering...

10 October 2018 7,691 4 View

How do I prepare an RNA gel?

I am looking to assess the quality of some RNA that I have extracted from human adipose tissue. Currently using a page supported by ThermoFisher...

10 October 2018 4,787 0 View

Are my adipose tissue samples still viable after being in a -80 that went down on us over the weekend?

I have some human adipose tissue samples. -80 C went down on us over the weekend. We are unsure of how long the freezer was actually down for. When we got it back on the outside thermometer on the...

08 August 2018 6,546 6 View

How do I design primers/probes for eukaryotes?

I am seeking assistance in regards to designing primer/probe sequences for eukaryotes (human). I usually go to NCI Gene --> enter the gene of interest --> click on FASTA sequence -->...

05 May 2018 5,070 2 View

What is the amount of RNA (adipose tissue origin) used for 1-step RT-qPCR?

I am working with human adipose tissue and want to quantify the expression of a few genes. My intent is to use a 1-step RT-qPCR protocol/kit to do this. I am not able to find information on this...

05 May 2018 5,590 3 View

What is the best way to store protein extracted from adipose tissue samples after QIAzol homogenization?

I will be extracting mRNA, miRNA, and protein from human adipose tissue using the miRNeasy mini kit and the RNeasy mini elute cleanup kit. After phase separation I want to store the proteins...

04 April 2018 4,473 3 View

What is the best kit/reagent to use for extracting total RNA and miRNA from human adipose tissue?

I will be extracting mRNA and miRNA from human adipose tissue. In your experience, what have you found to work the best?

03 March 2018 2,560 4 View

Issue extracting RNA from adipose tissue

I am having issues extracting RNA from adipose tissue. Currently, I am using the miRNeasy Mini Kit from Qiagen following their protocol. The result is a low concentration and an A260/A280 of...

01 January 1970 9,400 2 View