Hi everyone,
According to your experiences, what are the best RNA extraction kits for FFPE tissue samples in terms of RNA quantity, quality, and process time? The downstream application will be RNA sequencing.
Thank you.
If your lab has money, the Promega CSC/RSC kit for RNA FFPE extraction is the best. We have evaluated many column, bead, technologies and many vendors, and the Promega routinely outperforms as far as sample quality, quantity, and purity. If no money, any home-brew deparaffinization methods (xylene, mineral oil) will work, coupled with your standard Trizol extraction will yield superb quality and quantity of RNA. Best of luck!
Dear Craig Silver could you possibly send your method for using xylene, mineral oil coupled with Trizol extraction.
I have worked with Amoydx FFPE RNA extraction kit, it gives good yield and quality RNA other than any other silica column based kits.
We have had good result with RecoverAll kit from Qiagen. The RNA sequencing (Quantseq, Lexogen) went well.
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