Hi Rangsun, one option is a program called BioEdit which runs natively on Windows.

http://www.mbio.ncsu.edu/bioedit/bioedit.html

I'm currently using it for my sequencing and while it's not as intuitive as Sequencher for example, it's great for freeware. It is possible to run it on a Mac as well, it just takes a bit more fiddling around to get it installed. I've placed the link to the Mac OSX install instructions below if you want it.

https://phylogeneticgeek.wordpress.com/2011/08/05/how-to-install-bioedit-on-a-mac/

Thank you very much Ricardo.

try with MEGA

Artemis is another good one - 

Get BioEdit. It can do lot more than any other software and is free. It might take some time before you get used to it, but it will be a life saver later. There are some tutorial videos on youtube to get started with bioedit.

I recommend SeaView. Reads and writes several alignment formats, translates nucleotide to protein sequences and can colour nucleotide sequences by codon (very helpful for in-frame alignment). Also has in-built alignment and ML tree estimation tools. Works well on Mac, Windows and Linux

http://doua.prabi.fr/software/seaview

Many thanks for all great suggestions.

GenomeCompiler is a goodone and is also free!!

mega works in mac as well

A plasmid editor (ApE) works great.

Many thanks for all great suggestions.

I think as first you can go with Bioedit. Simple and easy to use. Moreover, you can find many videos in Youtube on how to use it.

I think is BioEdit

I tried installing all the options recommended by various users here, ApE (A plasmid Editor, http://jorgensen.biology.utah.edu/wayned/ape/ is easiest to download, install and use. Artemis is a Java application which requires me to install new Java, remove old Java, ... , takes forever on my PC and still not working properly. Bioedit is easy to install, but could not import GenBank entry via Accession number (complaining about URLs being too long). SeaViewis is flagged by my anti-virus TrendMicro as Ransomware suspect, and I dare not to allow it to be installed.

I'm a big fan of Serial Cloner. You can simulate PCR, digests, ligation, gateway cloning, etc. The alignment window is good if you're only aligning 2 things. I supplement that with SnapGene Viewer for visualizing primers and because it's prettier.

Can i get a professional to help me out with my sequence editing. You can reply my email.so we talk it [email protected]

Sapio Sciences Exemplar Electronic Lab Notebook (https://www.sapiosciences.com/electronic-lab-notebook-software-eln) bronze edition is free to academics and is a full featured ELN.

BioEdit does not seem to work anymore. Scrolling is not functional and it is impossible to look at long alignments. Is anyone having problems too? I tried to intsall it again but the original link is not available anymore. Other links allow downloading but I get the same bug.

Alexandra Hiller It still works for me. Did you try to re-download BioEdit from another resource?

Hi Amro Hashish, thank you for your reply. It is a weird thing because this problem started last night when I downloaded a sequence from GenBank, the same way I have done this for years. I tried everything possible, downloading again from the available sources, transferring the software from my old computer, where actually BioEdit still works. As soon as I try to execute the software in my computer the scroll bar stops being functional and therefore the bars to move up and down and along the alignment is not shown. Very frustrating because this is the best free program for cleaning and editing sequences and for aligning protein-coding sequences. I have used it for many years :(

I have same problem with BioEdit Alexandra Hiller

I have the same problem too.. i wonder if its something to do with only windows 10 pro. Is it possible?