Dear all,
We are planning to study plant volatiles in crop plants with and without aphid infestation in the field. My questions are:
(1) Which headspace sampling setup would you recommend?
(2) Which material should be used to capture the volatiles (e.g. charcoal filter)?
(3) At which temperature should we store the samples (-20 or -80°C)?
Thank you very much!
Christoph
Dear Christoph,
I advise you to have a look at this paper:
Kallenbach et al (2014). A robust, simple, high-throughput technique for time-resolved plant volatile analysis in field experiments. The Plant Journal, 78, 1060-1072.
I found this paper very inspiring when I did VOC analyses during my PhD.
Cheers,
Benjamin
Dear Christoph,
Pls. find the attached files,
Hoping this will be helpful to your project, good luck
Regards
Prof. Houda Kawas
https://link.springer.com/article/10.1007/s10886-015-0559-9
http://agris.fao.org/agris-search/search.do?recordID=US201400035476
http://www.bio-protocol.org/e1391
http://s3.amazonaws.com/academia.edu.documents/43582906/A_review_of_advanced_techniques_for_dete20160310-15534-xb1fbn.pdf?AWSAccessKeyId=AKIAIWOWYYGZ2Y53UL3A&Expires=1498814812&Signature=tpKz6%2Bo2J16nb%2BKsq3JPfeL9ZpU%3D&response-content-disposition=inline%3B%20filename%3DA_review_of_advanced_techniques_for_dete.pdf
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2005.02612.x/full
Article Real-time monitoring of herbivore induced volatile emissions...
Hi Christoph
You can find several references on attachment 1
https://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2005.02612.x/pdf
6
https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-6-6
8
https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-6-6
17
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1025.9395&rep=rep1&type=pdf
19
https://www.agilent.com/cs/library/primers/public/5991-3326EN_SPHB.pdf
- Badges
- Science topic
- Similar topics
- Biological Science
- Zoology
- Invertebrate Zoology
- Entomology