Hi. Is these pictures of fish liver? I am better with the fish gills histological changes, but I think I can see hepatic vacuolation and hepatocytes with karyopyknosis, karyorrhexis and karyolysis.

https://www.researchgate.net/publication/269574874_Biomarkers_in_European_perch_%28Perca_fluviatilis%29_liver_from_a_metal-contaminated_dam_lake

https://www.researchgate.net/publication/267042819_Metal_effects_on_histological_and_biochemical_parameters_of_common_rudd_%28Scardinius_erythrophthalmus_L.%29

Article Biomarkers in European perch (Perca fluviatilis) liver from ...

Article Metal effects on histological and biochemical parameters of ...

hi, no , rabbits liver

Hi

I believe these sections represents adenocarcinoma of the liver. The glands are clear in pictures 3 to 5. There is also vaculation and displasia of the hepatocytes (loss of hepatic architecture) in pictures 2 and 4 suggesting carcinoma of the liver.

Is this a model of adenocarcinoma with metastasis in the liver?

The attached photos are from metastasis of colon cancer in the liver of rat.

Hope this is helpful.

Bassem

You can perform a reticulin-stain for better diagnosis. In hepatocellular carcinoma the reticulin-fibers are typically changed.

For human liver there is a antibody called Glypican-3, that is specific for HCC.

To complete the interpretation you should also perform PAS and Trichrome-stain. This gives you information about the glycogen-content and the grade of fibrosis.

The vacuolation in 2 and 4 would be compatible with glycogen. There is also mild lymphoplasmacytic peri-portal infiltrates in 3, 4 and 5. There seems to be mild periportal fibrosis also in 3. The trabeculae in 6 seem atrophic. Picture 1 looks pretty normal, with only minimal glycogen.

The stain is quite different on these slides (variable eosin and hematoxylin intensity), so I suppose these are all different cases.

Dear Mon I

You need to provide as much signalment, clinical signs and clinical history as possible to expect useful diagnoses for these cases based on limited images.

Kind regards

Derek