Dear Colleges,
I was wondering about the best method to prepare plant tissues for cryosectioning avoiding traditional chemical fixative methods. I hear about cold isopenthane method as on of the most indicate way to prepare the samples. But, when we discuss about the cryofixation of samples to big and delicate as flowers and fruits. It seems a huge challenge to get access to internal tissues that has a high amount of intercelular space. Does anyone knows about some alternative cryofixation methods?
Regards,
João
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