What is the best concentration of proteinase inhibitor cocktail to preserve demineralized bone matrix?

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Steingrimur Stefansson

Hi Luz, as far as I know, bone has a lot of proteinases, but you will need a proteinase inhibitor cocktail that also has EDTA.

I would try the Roche cOmplete first.

http://lifescience.roche.com/shop/products/complete

Luz Elba Lopez Alvarez

Thanks Steingrimur, I will check the Roche products.

Best

Luz

José Francisco Gómez-Clavel

Hola Luz. puedes usar 1mM de Fluoruro de metil-metano sulfonil y 1mM de N-etilmaleimida, 25 mM de EDTA y un microgramo/ml de leupeptina.

Muchas gracias por la información Dr. Francisco. Le deseo un feliz año nuevo, lleno de exito y salud.

Luz López