I will test cytotoxicity of a drug on cancer and normal cell lines then iam planning to make immunocytochemistry, what are the concentrations can I use for immuno papers differ in this point
The recommended conc should be included in instructions manual. The same antibody may work differently in different tissues at the same concentration Start with the lowest conc that can give positive reaction. I recommend you to use different conc at the same time and detect the suitable one.