What is the best cell line module to study Non Alcoholic Steatohepatitis?

More Muayad S Rahma's questions See All

Reconfigurable Intelligent Surfaces?

I have an idea related to Reconfigurable Intelligent Surfaces In RIS technology, waves are reflected. Can we do the opposite? I mean that we collect or attract these waves instead of reflecting...

18 January 2024 8,550 3 View

Are there any difference between Reference gene and Calibrator gene in calculating the relative quantification of RNA expression?

I'm self-studying RNA expression, and I'm quite confused with the difference between Reference gene and Calibrator gene in calculating the relative quantification of RNA expression. Furthermore,...

21 November 2023 6,296 2 View

Coupling Thermal-Electric and CFD Simulations in Ansys?

Hello everyone, I am currently working on a challenging task involving the coupling of Thermal-Electric and CFD simulations within Ansys. The issue at hand revolves around a material with a...

20 August 2023 2,611 0 View

Is it possible to consider that consciousness is a kind of energy ?

Can we consider consciousness as a type of energy that supplies the brain with energy, given that the brain has very high resistance, up to 60 ohms, and in order to generate small currents within...

09 August 2023 8,142 10 View

How to Merge Faces of STL file to one surface without reducing the quality of the geometry ?

Hello everyone, I am currently working on a case involving Cardiovascular disease using FSI (Fluid-Structure Interaction). I have an artery in an STL file. However, when I created a shell for the...

10 July 2023 5,564 6 View

I need help with my study?

can you help me find local and international literature on incivility in the workplace among nurses? please help

22 June 2023 7,197 0 View

If consciousness were simulated, would there be a conscious AI?

If consciousness is simulated, such as simulating a neuron, will there be conscious artificial intelligence, certainly not like human consciousness, but conscious in another way?

19 June 2023 6,997 3 View

How does consciousness arise in the brain through ions in neuronal membranes ?

I have an idea to explain consciousness. About 8 years ago, Professor Donald Hoffman asked a very important question to help us understand consciousness. He asked, "How is it possible for calcium...

16 June 2023 6,204 4 View

Could people who habitually take antihypertensive drugs such as losartan, be given a class of antihypertensive medication, such as candesartan?

Antihypertensive Medications - StatPearls - NCBI Bookshelf (nih.gov)

07 June 2023 226 3 View

Is it possible to publish research on an idea to develop artificial intelligence, or should a model be made first for this idea?

13 May 2023 2,368 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Ethylene glycol is newtonian or non newtonian fluid?

Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.

09 August 2024 2,111 2 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Javier González-Gallego

You could use HuH7 human hepatocytes incubated with methionine-choline deficient medium. See our paper http://www.ncbi.nlm.nih.gov/pubmed/25712622

Lirui Wang

Thanks. Sounds a good idea.

Jianbo Jia

Oleic acid induced hepatocytes (HepG2, Bel7402 etc.) or oxLDL induced hepatic macrophages might be taken into consideration.

Could you explain how oxLDL works?

Muayad S Rahma

Thanks for all the answers, I am thinking to use HepG2 and LX-2!

Joshua Samsoondar

Primary mouse hepatocytes would work as well, and you avoid having to use cell lines