What is the best cardiomyocytes: cardiofibroblast co-culture ratio for 2D experiments?

10 February 2013 1 6K Report

Also, what is the suitable concentration of cardiomyocytes to take an effective beating with 2D samples?

Depends on your differentiation, but with EB's we've seen cardiomyocyte containing clusters beat with as few as 3% of the population as Nkx2.5+ myocytes.

Cheers

John

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Seda Nur Topkaya's questions See All

Has anyone faced low transfection efficiency of SK-BR-3 and BT-474 cell with Lipofectamine 3000?

Hello. I've been trying to transfect plasmid DNA to my breast cancer cell lines (BT-474 and SK-BR-3) with Thermo Fisher's Lipofectamine 3000 reagent. According to the thermo fisher, those cells...

24 March 2024 9,489 5 View

Why are there so many deviations in my absorbance values in the MTT assay?

In the MTT assay, I read the absorbance values at 570 nm. However, sometimes there are so many deviations in the absorbance values. Hence, I have to take out some (up to 4 values) deviated values...

13 November 2023 4,928 0 View

Betwenness Centrality in Structural Connectome?

Hello everyone! I extracted betweenness centrality values of more than 250 data by using number of streamline weighted connectivity matrices. In order to calculate the betweenness centrality, I...

18 June 2023 4,839 3 View

Why can't I print tissue scaffolds properly?

Hello, I've been working with a 3D SLA printer to produce a bone tissue scaffold and trying different parameters, both the ceramic slurry and the 3D printer. However, I haven't achieved successful...

07 February 2023 3,169 6 View

How can I dissolve glyphosate?

I try to dissolve glyphosate in water with heat and without heat. I read an article that they mentioned using isopropanol or ethanol to dissolve glyphosate, but they did not explain the...

02 November 2022 5,294 3 View

How can use machine learning with the family data?

Hello everyone, I am planning to design a model that can predict individuals with high or low psychosis-proneness by looking at the brain connectivity measures in 114 regions, age, gender, IQ,...

12 September 2022 5,046 1 View

I have values from -4.5 to 10 in my database, and it is not normally distributed. Is there a valid way to transform the data into positive values?

I have one environmental risk score for psychosis (ERS) value for each participant in my database. The scores vary from -4.5 to 10. And the data is not normally distributed. I would like to ask...

29 July 2022 9,980 3 View

SacB counterselection troubleshooting?

Hi! I'm trying to create a deletion mutant using pEX18 Gm. I amplified my gene, cloned into pEX18 and with electroporation (1600v), my plasmid vector integrated into P.aeruginosa genome. But now...

12 March 2022 9,766 3 View

Specific gene region scanning?

Hi, I have the total genome sequence of an organism. I want to scan for the presence of a special gene from this sequence. How is it done, with which program should I do it? I would be very...

26 November 2021 6,206 3 View

How to extract FA and MD values for each participant if new data are added every week?

Hello Everyone, I have overall 150 data, and my professor asked me to extract each participant's FA and MD values. I know how to get mean FA and MD by using TBSS in FSL. However, since we are...

14 July 2021 1,759 4 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View