Dear colleagues, I am looking for a positive control for proliferation assay in Beas-2B?
Thankx
I’m a graduate student and I’m going To start my thesis research , could you help me to find something related to the islanding detection to research about ,thank you at all
06 January 2021 6,694 5 View
Has anyone experienced an increase in the absorbance reading with increasing concentration of a substance with expected antioxidant effect using the DPPH assay?
10 July 2019 3,734 3 View
Hi all, I am trying to identify flanking sequence for insertion site, using adapter method, so since I check the protocol, the master mix of the PCR reaction includes: 5x cresol-red glycerol,...
07 January 2018 5,738 4 View
In USA and Europe, wrestling and boxing have a wide popularity, like UFC and WWE. Does watching violence reduce the aggressive in human been ?
29 May 2017 8,074 81 View
Hi I tried the Sigma's recommendations (3.3 mg/ml) it didn't work so I went down to 1.9 mg/ml and I still can see the particles of the powder. I read something about using 1% ascorbic acid but I...
15 May 2017 7,256 2 View
Some specifications recommend testing cylinders, others recommend testing cubes !
28 February 2017 8,154 23 View
In prestressed concrete, additives always are used. There are different types of them. There effects should be known.
27 February 2017 864 15 View
1. comparative contingency theory with other theories. 2. is the contingency theory adequate for management accounting research. 3.can the management accounting practice improve the...
16 February 2016 7,212 4 View
I find controversial data on the primers used in amplification and sequencing the promoter and all exons 1-5, can anybody help?
12 July 2015 8,498 1 View
Shimadu LCMS-8040 LC/MS/MS System i need an application for analyzing peptides and amino acids for my research
06 July 2015 2,178 9 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View