Hey!

I used to activate them with PAF and always I got activation by secretion of EPO after 1hour.. also I used SEB (Staphylococcal enterotoxin B) to activated them.. you can check or EPO release or cytokines release.. both are working for me..

Good Luck..

Thanks! How do you measure EPO? With an ELISA kit?

You are welcome! we measure EPO by an enzymatic assay.

Would you be able to share a protocol for it?

What cytokines do you look for? IL-5, IL-4, IL-13?

Also at what concentration do you use PAF to stimulate eosinophils? And do you stimulate them for 1h only? Thanks!

yes sure, you can look for IL-5 and IL-4, I don't know about IL-13, I used both 10uM and 20uM, yes for 1h its enough.

you welcome :)

you can find it here,

and sorry for the late replay.

good luck.

Dear I support PEF and SEB and EPO is measured using ELISA kit. You can use Elabscience kit.