How to quantify or determine success of Enzymatic PEGylation?

Hi, my name is Rossana and I'm a PhD student in Bioengineering. I'm running into the topic of enzymatic PEGylation, but one of my biggest concern is: once you have designed your experiment and...

13 October 2020 5,039 4 View

How to export data from static analysis in Ansys Workbench to excel?

Hello, I am running a static analysis for 10 time steps (variable load) in Ansys workbench 19.2. I would like to export to excel the nodal stress everywhere in the structure (not just the maximum...

17 August 2020 6,105 3 View

Who is accepting proposal for publication in research journals?

I would like to submit a research manuscript about the education of transnational students between Mexico and the U.S to be reviewed for publication. Therefore, which education research journals...

13 July 2020 4,649 3 View

I need to change the citation style of a article made using MS word Puglin. I tried to use Mendeley guide, but it didn't work! Anyone could help?

I tried Mendeley support too.

06 March 2019 2,728 3 View

How to obtain bending and torsion modes of vibration in Ansys APDL?

09 February 2016 9,334 3 View

Are space groups H3 and R3 the same?

If it is the same, which is the right nomenclature H3 or R3? I would like to know the advantages from a crystallographic point of view of using an hexagonal representation? Any other explanation...

14 April 2013 8,497 4 View

Number of reflections vs. symmetry and resolution

I have a crystal with lower symmetry (orthorombic) that was measured at higher resolution than another crystal (hexagonal) and the number of reflections from this first xtal is lower. In the...

14 April 2013 939 11 View

Repulsive interactions in a protein-protein complex, or wrong estimation of interactions?

I scored the interactions in a protease-inhibitor complex using the 3D crystal structure, and I found these contacts involving two basic residues: Arg18(NH2)-Arg61(NH1)...

24 November 2012 3,306 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View