My lab is ordering a setup to perform electrophysiology in mice and I would like to know the general advantages of adapting the system to work with digital electrodes or headstages.
I only have this enzyme in the lab to promote in vitro transcription. I know I should get another DNA template subcloned with a SP6 promoter, however I am very curious to know if SP6 RNA...
03 April 2014 6,047 4 View
The publishing world is rapidly changing, and the leading titles are facing increasing competition. Instead of publishing in established journals, would you take the chance to publish in journals...
03 April 2014 10,006 0 View
I have got a gene that I want to cut out of its present vector and ligate it into another vector, but the stop codon is the problem. Any suggestions?
10 November 2012 8,215 10 View
At some point in drug development of kinase inhibitors, we should know if the drug can bind (at similar affinities) to the both phosphorylated and un-phosphorylated forms of kinase proteins .
09 October 2012 6,353 8 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
04 August 2024 4,090 0 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View