Luiz Alexandre V Magno

5 Questions 12 Answers 0 Followers

Questions related from Luiz Alexandre V Magno

What is the best advantage of using digital versus analog electrodes for in vivo electrophysiology? Is it worth it?

My lab is ordering a setup to perform electrophysiology in mice and I would like to know the general advantages of adapting the system to work with digital electrodes or headstages.

10 October 2018 9,269 0 View

Does anyone know if SP6 RNA polymerase works on DNA templates under a T7 promoter?

I only have this enzyme in the lab to promote in vitro transcription. I know I should get another DNA template subcloned with a SP6 promoter, however I am very curious to know if SP6 RNA...

04 April 2014 6,015 4 View

Would you publish your paper in a scientific journal that has been launched recently?

The publishing world is rapidly changing, and the leading titles are facing increasing competition. Instead of publishing in established journals, would you take the chance to publish in journals...

04 April 2014 9,986 0 View

Can anyone suggest a way for removing the stop codon to make a fusion protein?

I have got a gene that I want to cut out of its present vector and ligate it into another vector, but the stop codon is the problem. Any suggestions?

11 November 2012 8,192 10 View

How could we evaluate (in vitro) if phosphate modifications at specific sites in proteins affect drug binding?

At some point in drug development of kinase inhibitors, we should know if the drug can bind (at similar affinities) to the both phosphorylated and un-phosphorylated forms of kinase proteins .

10 October 2012 6,319 8 View