What is the basics of using a reference wavelength in calorimetric assays?

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Kong M Li

Hi Shinsmon, for colorimetric assays, the purpose of using reference wavelength is to correct/normalize any changes that is not from the measuring wavelength of your analyte, such as well to well variation in ELISA plate etc . Basically the reference wavelength should be close to the measuring wavelength but no overlapping with the spectrum of the analyte (always good idea to obtain the the analyte spectrum to determine the reference wavelength).