What is the anatomical site on E. coli responsible for release of alkaline phosphatase, DNases and penicillinase?

30 August 2018 1 4K Report

An aliquot of E. coli was treated with EDTA. The first wash was analyzed and found to contain alkaline phosphatase, DNases and penicillinases.

Michael J. Benedik

Those are all periplasmic enzymes, so it is not a surprise that when you treat with EDTA you disrupt the outer membrane and the contents of the periplasm are released.

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