Is Granzyme B secreted when the NK cells gets activated or it only secrete when it gets in contact with the target cells?
Both NK and CTL cells require contact with their target cells before they release the PF/GB lethal combination
Hi Lillian,
Thank you for the answer
I am planning to do ELISA assay for Granzyme B using cell culture supernatant obtained from the coculture of Killer cells and Target cells, do you think it is suitable.
The only problem I can for see is if you miss the time point and GB goes into the target cell you might not get the best read out? You might have to do various time points at pretty close intervals to optimize for your assay conditions.
Alternatively, you could also back it up with flow cytometry or immunocytochemistry; these assays will allow for intra-cellular staining and a better idea of how much GB is within the cells
Thank you Lillian
I will combine both Flow and ELISA to measure and detect GB.
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