My antenna is circularly polarized. I want to know whether it is LHCP or RHCP. So, I plotted Gain RHCP vs frequency and Gain LHCP vs frequency graph. I am getting gain in negative value for both cases. Is it acceptable?
I expect that your gain is is dBi, which is decibels relative to an isotropic radiator. If this is negative it means you have less gain than an isotropic radiator. This often happens, if your antenna has a very wide beam, and is not very efficient. If your antenna has a narrow beam and has negative gain then it is either very inefficient or you have made a mistake.
If you don't know what decibels are or don't know what an isotropic radiator is, look them up on the internet or in a textbook.
If your antenna is linearly polarised then RHCP and LHCP will both be 3 dB less than the peak linear gain. I can't think of another case where both will be lower than that. As both are more than 4.4 dB lower than the peak gain then there must be something wrong somewhere, because I don't think this is mathematically possible for a real antenna.