Recent experiment on photon as both wave and particle, what do you think is it both wave and particle or it depends upon experiment you choose?

04 May 2015 5,868 7 View

How does gravity affect the Schrodinger wave function?

How does gravity affects Schrodinger wavefunction?

02 March 2015 7,173 14 View

If we give a name to a particle and explain its properties does it explain everything about it?

If you consider electron (any particle), it is understood by Dirac equations. We understand all of its known properties. But we have no idea what are electrons (actaually any partticle or bosons)...

01 February 2015 6,333 21 View

Why Dark matter and Dark Energy not part of fundamental forces?

I know fundamental forces are made up of 4 forces Gravity, Electromagnetic, Strong and Weak forces. Why Dark matter and Dark Energy not part of fundamental forces? Is it because we don't know...

31 December 2014 5,031 8 View

Do electromagnetic tensors fail to explain imaginary components of electric and magnetic field?

31 December 2014 9,854 7 View

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31 December 2014 2,904 7 View

I asked CERN to conduct experiment on Dark matter, they told me to get affiliated with any university affiliated with CERN, is this important ?

I contacted CERN couple of months back to conduct an experiment which will help whole world to understand what are dark matters. Reply was good (because this is only organization which replied),...

09 October 2014 7,453 0 View

If the positronium minimum radius is equal to the Bohr radius, does that mean our space is quantized?

Positronium annihilates even before the lectron and positron meet. Does that mean that our space is quantized (like it is in holographic plates in form of bit)? Regards, Bhushan Poojary.

09 October 2014 2,460 17 View

Which experiment can determine if our universe is hologram or not?

Which experiment can determine if our universe is hologram or not? Is it confirmed yet or still is it an open question? Regards, Bhushan Poojary.

09 October 2014 3,365 12 View

What happens when electron passes through matter wave?

08 September 2014 5,281 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View