7 Questions 14 Answers 0 Followers
Questions related from Mangesh Kshirsagar
While preparing agarose gel, if we add Agarose powder in already boiled 1X TAE buffer, clumps (precipitation) formation observed. Agarose was not getting dissolved uniformly even after mixing and...
13 February 2017 9,659 1 View
I have cloned gene in one plasmid and sequence confirmed with Miniprep. I want to scale up same plasmid, for that I transformed my Final Miniprep plasmid into DH5 alpha cells and further used a...
20 December 2016 7,171 4 View
Sometimes, after plating transformed bacterial cells, colonies grow on plate are of different diameters. What are the factors responsible for uneven sizes of colonies on plate?
13 August 2016 1,142 5 View
If the same plasmid with same insert is used for transformation into DH5 alpha (E.coli) and Stbl2 (E.coli) and if further done scale up. What will be the qualitative and quantitative difference...
07 June 2016 2,317 4 View
I am trying to linearize one circular plasmid with NEB NruI restriction enzyme. I have sequenced my plasmid and confirmed the NruI restriction site. I am using NEB buffer 3.1 in the NruI digestion...
07 June 2016 6,592 4 View
If the number of colonies are very high (even hard to count on plate) when transformation done with 1 ng of DNA then how to calculate transformation efficiency? Anyone experienced that even with...
04 June 2016 7,895 4 View
I am getting nonspecific bands on SDS PAGE after digestion with factor Xa. Is there any alternate cost effective method/component for factor Xa?
26 August 2015 2,849 3 View
