What exactly is probe resolution and how can a probe resolution be used for partial overlapping tilling array used for CGH analysis?
I have a .text file for various FASTA sequence , and i want to convert these sequences into a numeric file which will be in .csv format. OR I want to extract physiochemical properties(features)...
25 July 2024 3,650 2 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
As the biaxial loading may implement additional hardening of the material during fatigue with an increase in the biaxiality ratio. What would be the effect of planer biaxiality on dynamic strain...
31 May 2024 9,104 0 View
I have just started working on fpga and as a task I need to establish communication between 2 basys 3 (xc7a35tcpg236c-1) boards using usb uart bridge. What should be my approach for this? Like for...
05 May 2024 447 0 View
Hello All, I want legends to be positioned below the graph (outside), but in two or three rows accordingly, such that they do not extend beyond the vertical boundaries of the graph. Can anyone...
23 April 2024 7,447 1 View
Ethics
15 April 2024 6,778 1 View
Hello! I want to know how to test the electrochemical stability of the electrolyte (2M ZnSO4 solution) I have measured electrochemical stability window of a electrolyte by 3 electrode...
26 March 2024 3,306 1 View
Dear all, Will you please let me know how to calculate the carbon, nitrogen, and phosphorus (C: N:P) ratio in wastewater? Is it advisable to measure total COD, nitrogen, and phosphate? or I...
05 February 2024 4,105 3 View
When OCP stabilizes in a given electrolyte there is formation of passivation layer on sample surface or it shows that at this potential corrosion occurs. Also my tafel Ecorr values are not comming...
04 February 2024 8,735 2 View
Stage Temp Time Temp/time 1 95°C 5min - 2 95°C-85°C -...
31 January 2024 1,925 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
AGILENT HPLC 1100 SERIES operation assistance needed.
25 July 2024 10,045 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View