Isha Pandey

6 Questions 12 Answers 0 Followers

Questions related from Isha Pandey

Can anyone share protocol of expressing intrensically disordered protein?

I have been trying since long to express two proteins of eukaryotic origin in E.coli but somehow these proteins are not showing any expression. Even i have checked the transcript level transcripts...

02 March 2017 4,603 4 View

What is overall median probe spatial resolution?

What exactly is probe resolution and how can a probe resolution be used for partial overlapping tilling array used for CGH analysis?

31 March 2014 433 0 View

How to confirm single digestion or double digestion?

Is there any technique by which I can confirm that after doing a double digestion of a vector it is completely double digested by both the enzyme or only it is digested by single enzyme? How...

22 October 2013 1,857 4 View

How to calculate average log ratios of probes?

Average log ratio

29 September 2013 1,322 2 View

What is Amplification and deletion call in terms of a CGH data?

I am analyzing array CGH data, and I am not be able to understand the amplification and deletion call what they represent in terms of Gain or loss of a particular gene.

25 July 2013 8,665 0 View

Is it possible to see a dimer of protein in a SDS page even after using Beta mercaptoethanol and heating the samples before loading?

Hi there, I am trying to express a protein of approximatly 60 kda, but sme how i am not getting band at desired size but i am getting band just double of the desired size. so my question is, that...

01 January 1970 3,239 12 View