What is On-off cycle ratio of compressor in 1 ton AC?

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What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

28 March 2024 259 3 View

Why do I get minor electric shock in my laboratory when touch something metallic?

Sometimes when I touch my colleague or metal bodies. i fell shock with some minor electric sound.

27 February 2024 1,191 3 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

How to do multiple data sequential Rietveld refinement in TOPAS?

I can do Rietveld refinement of single multiple phase data, but I am not able to do multiple data sequential Rietveld refinement. I am using Topas V7.

21 January 2024 2,083 0 View

Why does the transmittance % in FTIR become more than 100% (Opposite direction)?

I did FTIR of a reactive dye's powder. But I see that the transmittance of 3 peaks went opposite direction of normal peaks and eventually the transmittance percentage became more than 100%. What...

17 December 2023 7,093 2 View

Does a thinner intrinsic layer in a 'p-i-n' solar cell, compared to the diffusion length, lead to increased or decreased charge carrier recombination?

As we know, when the thickness of any layer in a p-n junction solar cell exceeds the carrier diffusion length, the photo-generated carriers recombine before reaching the charge transport layer....

15 December 2023 4,930 3 View

How to calculate polymorphic information content (PIC) value for SCoT marker data? Which software helps to calculate it?

I am trying to evaluate the genetic homogeneity of wild grown mother plant and tissue culture raised plants of Piper sp. using SCoT and other dominant markers.

06 December 2023 1,837 0 View

To generate the temperature map of a city, what resolution shall be efficient way of representation (eg:500sq.m or 1sq.km grids) ?

We have a telematics data - temperature values are collected per second. To generate a hourly map of temperature of a city, suggest the appropriate resolution. This map is not for any specific use...

20 November 2023 9,954 1 View

Does enthalpy of chemical sorption reaction is a strong function of pressure and/or temperature for soprtion of ammonia in halide salt?

In most of the literature the enthaply of formation/reaction is chosen to be an constant. Some researchers claimed that in a given pressure range (say: 10 bar) this parameter can be chosen to be a...

07 November 2023 8,839 0 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

How movement of energy in an ecosystem different from chemical cycling & movement of matter through an ecosystem differ from movement of energy?

How are the movement of energy in an ecosystem different from chemical cycling and movement of matter through an ecosystem differ from the movement of energy?

03 August 2024 6,134 0 View

Why does the flow of energy through the biosphere depend on the cycling of nutrients and matter and energy flow across the earth's subsystem?

03 August 2024 6,412 0 View

How does matter cycling and energy moving through the ecosystem differ from one another and cycling of matter through the ecosystem?

03 August 2024 2,558 1 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?

31 July 2024 3,965 0 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?

31 July 2024 8,483 8 View

How do bacterial species contribute to ecosystem functions and nutrient cycling and role of microbial communities for sustainability?

25 July 2024 2,351 2 View

What role do microorganisms play in the cycling of matter and role of microbes in the biogeochemical cycle?

25 July 2024 3,096 2 View