Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

How many GCMs exist currently? Which are the most prominent ones among them?

General Circulation Models or Global Climate Models are over 40 when I went through a research article long back. I would like to know how many GCMs are currently in use. Names of them aren't what...

03 August 2020 2,789 5 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

Is groundwater content considered in SM2RAIN algorithm?

I have come across SM2RAIN algorithm and can't help but wonder about groundwater content. I couldn't notice its inclusion in the algorithm. Is it something I've missed or I need to refer further?...

09 June 2020 5,272 6 View

How to do regridding using MATLAB?

I have a batch of netCDF files of precipitation data at 30km * 30km resolution. I attached a sample file of it. I want to convert them to 10km * 10km resolution using MATLAB. I tried using...

12 May 2020 6,007 6 View

What are the uses of extreme values in Hydrological aspect?

For the research work I'm working on, I came across usage of extreme values while studying about deriving IDF curves for future climatic scenarios. What kind of results can I produce with extreme...

27 January 2020 4,145 4 View

What are the alternatives of Global Climate Models? Apart from downscaling, any methods exist to derive local climate variables from GCMs?

GCMS are used to predict atmosphere and climate behavior. Apart from GCMs, are there any methods to predict climate behavior? In GCMs particularly, are there any methods to derive local-scale...

02 January 2020 7,752 14 View

What the concentration should taken of Traut's Regents and Peptide?

i am coupling 31 amino acid sequences to PEG, before that i have to activated this peptide with traut's reagents. Can anyone suggest me the which molar equivalent of concentration of both chemical...

07 April 2019 6,334 1 View

How to perform Cytotoxicity activity of Plant Extract (essential oil) without taking of 100% DMSO?

My super critical fluid extracts (SFE) are immiscible in 1 % DMSO for carrying out Cytotoxicity assay SFE is essential oil so i have problem to mix the oil and 1 % DMSO due to different polarity...

20 March 2019 2,037 2 View

Can anyone suggest me method for the coupling of Peptide with DPSE-PEG-MAL?

1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine- N-[Maleimide (Polyethylene Glycol)2000] ammonium salt [DSPEPEG- MAL] is poymer and I want to conjugate with some peptide. So Please suggest me any...

13 November 2018 2,081 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View