What is the cause of colorful things?

01 February 2016 1,289 1 View

Can I use XRD to verify the inclusion of mesoporous silica nanoparticles in a hydrogel?

In part of my project, I build a matrix of hydrogel-silica nanoparticles. I want to know what types of test (such as XRD, FTIR, TEM, SEM, etc) I can use to verify silica nanoparticle inclusion...

01 February 2016 6,888 5 View

How XRD results (shift in observed peaks between different diffraction angle) can be related to cross-linking density?

01 February 2016 1,667 4 View

Does DAPI stain live and dead cells?

Does DAPI stain live and dead cells? I would be very appreciative if someone introduced me to suitable sources for usual markers (such as DAPI) and its protocol. Thanks

01 February 2016 8,273 6 View

What is sol content of a polymer and how one can measure that?

what is sol content of a polymer and how one can measure that?

31 December 2015 8,189 5 View

How can I set flux equality in a boundary in COMSOL?

I have a mass transfer problem and I wanted to set a flux equality (continuum) in a boundary in COMSOL package. I have two region and the boundary is between the regions. How can I do that?

01 February 2015 5,614 21 View

How can I find the partition coefficient of a particular drug in PLGA?

01 February 2015 3,808 4 View

How to schedule successive physics in COMSOL?

31 December 2014 968 6 View

Can anyone help with a question about exertion of basal metabolic rate in tropical areas?

In these areas, temperature and humidity is very high. how BMR is exerted in these areas? No benefit of temperature gradient and evaporation!

09 October 2014 9,179 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View