Maybe somebody can help me and say where is a problem with my methodology. I am fermenting R. leguminosarum in YEM broth. The growth is good, I can see a lot of cells under microscope, but when I am trying to determine the number of cells by plate count technique on the same medium with agar, the colonies do not grow. I tried incubate the plates in 26, 28 and 30 oC but the result was the same. What am I doing wrong?
Rhizobium leguminosarum is a aerobic bacterium. Do you use spread plate method? (Ar naudojamas pavirsinis sejimo budas?)
Dovilé:
have a look on these papers, may be you´ll find some helpfull information (e.g. concerning the C-source for agar growth of R. leguminosarum):
http://jairjp.com/JANUARY%202014/04%20VISHAL.pdf
Vincent, J. M. 1970. A manual for the practical study of root-nodule bacteria. IBP handbook 15. Blackwell Scientific' Publications, Oxford.
According to my experience is very important C-source type and N-source concentration.
Regards
Vit
Dear Vit Mateju,
Thank You very much for paper, I will examine everything! :)
Dear Mehdi Rostami Rad,
Yes, I tried to use YEMA with Congo Red. But the result was the same as using YEMA without congo red.
Dear Dovilė
follow this paper:
Egeraat AW. Growth requirements of Rhizobium leguminosarum, strain PRE. Plant and Soil. 1977 Aug 1;47(3):699-702.
Best
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology
- Microorganisms
- Bacteria