What is geltrex dilution for U87-MG in invasion assay?

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The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

What tools or software can I use in order to visualize EEG brain to brain synchrony?

I want to visualize brain to brain synchrony results (using EEG) as an illustration of two 3D heads with lines connecting the electrodes positions between the two heads where brain to brain...

16 November 2020 7,742 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

Silica gel in the sample on RP-HPLC ?

Hi Guys! Excuse my ignorance but I have little experience with RP-phases. I've managed to initially purify my peptide product using normal phase flash-chromatography. Unfortunately, Ihad to use a...

20 July 2020 7,274 14 View

Anyone knows what causes eye lid problem in Siberian hamster?

Several months ago we observed some strange changes around eyes of several individual Siberian hamsters (Phodopus sungorus) in our colony (outbred). I attach pictures. It looks like an...

17 July 2020 9,941 3 View

Is there any way to receive Hirsch index excluding self-citations from the Web of Science core collection?

The only thing I see is the Hirsch index value including all citations. Despite looking through the WoS tools I could not find any way to exclude it. However, I'm quite sure there must be some way...

15 June 2020 6,324 2 View

What is the scaling law for thermal damage to biomolecules/proteins from ultrashort heating?

It is well known that heating can denature proteins. However, what does happen to proteins in the case of short and ultrashort (microsecond or nanosecond scale) heating to extreme temperatures...

05 June 2020 6,650 1 View

Challenges in medical education in pandemic era?

These days many of academic teachers around the world have switched to e-teaching due to current epidemiological situation related to SARS-CoV-2. This is a great challenge to both students,...

31 March 2020 2,025 5 View

COVID-19 at psychiatric clinic?

In the current pandemic situation, it is not possible to completely avoid a psychiatric patient carrying COVID-19 or being directly symptomatic. In addition, medical priority is to help anyone....

19 March 2020 4,389 10 View

How to visualise cholesterol crystals inside tissue/cell?

Hi! I am searching for a method of visualisation/staining protocol for cholesterol crystals (not a free cholesterol, just crystalized) in cells as well as tissues? anyone has any experience??

04 March 2020 5,669 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

How long can you store Matrigel-coated Transwell inserts?

Hi, everyone! I was planning to do an invasion assay today and thus coated my inserts yesterday, but unfortunately, due to certain circumstances, I cannot proceed. The next time that I can do the...

09 February 2021 8,771 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Whic Type of Thermal Camera is Good to use in animal research?

Hello dear researchers I want to use a thermal imaging camera to livestock study. I wonder which type to use. If you know or used a good type please comment or insert its online link in...

29 January 2021 4,163 4 View

Cross-sectional area and mesh sensitivity in lattice modeling of RC structural elements?

In lattice modeling of RC structures, what is the mathematical concept behind choosing the cross-sectional areas for the simulation of, for instance, beams? Besides, seemingly, simulating the...

26 January 2021 2,638 2 View