In Gaussia Luciferase assay, the signal from coelenterazine decays really fast. In NEB's BioLux kit, there is a stabilizer to solve this issue. What is this stabilizer?
You can look for in following bibliography.
Verhaegen M. and Christopoulos T.K. (2002). Anal. Chem. 74, 4378-4385.
Tannous, B.A., Kim, D.E., Fernandez, J.L., Weissleder, R., and Breakefield, X.O. (2005). Mol. Ther. 11, 435-443.
Wu, C., Suzuki-Ogoh, C. and Ohmiya, Y. (2007). Bio Techniques. 42, 290-292.
I quote this from my published paper. "Twenty-microliter portions of the supernatant on the cocultured cells were assayed in duplicate for Gluc with 50 μl of 10 μM coelenterazine in 0.1 M Tris (pH 7.4) and 0.3 M sodium ascorbate". Please refer to PMID: 24049172 if you want further details
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