What are the anatomical borders of upper, middle and lower parts of rectum ampulla?

Hello, all. Could anybody give me link to any article or book in which clearly described anatomical borders of parts of ampulla of rectum. I found only ranges of these parts in cm.

09 October 2018 3,542 0 View

Is it possible to perform heterotransplantation of Erlich ascityc carcinoma (mouse) cell line from in Wistar rat?

Hello, all Is it possible to transplant Erlich ascityc carcinoma to Wistar rat?

08 September 2018 4,289 0 View

Can reviewer impose his point of view?

03 April 2016 223 4 View

What is relations between tumor associated B and NK-lymphocytes?

I made IHC and count number of tumor associated B and NK-lymphocytes in peritumoral zone of endometrial adenocarcinoma. Tau-Kendall analysis found direct correlations between this two kinds of...

31 December 2015 4,413 6 View

What is better open access or high impact factor?

Which journal is better for publication: with open access and free of charge but with low IF or high IF journal without possibility to publish open access (due to high charges)?

01 January 1970 333 5 View

Do you have any specific method of biomedical scientific articles reading?

Good day, dear collegues. When do you read different types of biomedical scientific articles (i.e. original articles, reviews, letter to editor etc.) at which moments you pay attention ? Do you...

01 January 1970 1,128 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View