Good day, dear collegues.
I am going to make immunohistochenistry for Galectin-1 at the immunostainer Ventana Benchmark GX. Does anybody have protocol for this antibody.
Good question dear
Hello, all. Could anybody give me link to any article or book in which clearly described anatomical borders of parts of ampulla of rectum. I found only ranges of these parts in cm.
09 October 2018 3,720 0 View
Hello, all Is it possible to transplant Erlich ascityc carcinoma to Wistar rat?
08 September 2018 4,414 0 View
Can reviewer impose his point of view?
03 April 2016 361 4 View
I made IHC and count number of tumor associated B and NK-lymphocytes in peritumoral zone of endometrial adenocarcinoma. Tau-Kendall analysis found direct correlations between this two kinds of...
31 December 2015 4,537 6 View
Which journal is better for publication: with open access and free of charge but with low IF or high IF journal without possibility to publish open access (due to high charges)?
01 January 1970 460 5 View
Good day, dear collegues. When do you read different types of biomedical scientific articles (i.e. original articles, reviews, letter to editor etc.) at which moments you pay attention ? Do you...
01 January 1970 1,247 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View