Hello, all
Is it possible to transplant Erlich ascityc carcinoma to Wistar rat?
Hello, all. Could anybody give me link to any article or book in which clearly described anatomical borders of parts of ampulla of rectum. I found only ranges of these parts in cm.
09 October 2018 3,720 0 View
Good day, dear collegues. I am going to make immunohistochenistry for Galectin-1 at the immunostainer Ventana Benchmark GX. Does anybody have protocol for this antibody.
01 February 2018 4,897 1 View
Can reviewer impose his point of view?
03 April 2016 361 4 View
I made IHC and count number of tumor associated B and NK-lymphocytes in peritumoral zone of endometrial adenocarcinoma. Tau-Kendall analysis found direct correlations between this two kinds of...
31 December 2015 4,537 6 View
Which journal is better for publication: with open access and free of charge but with low IF or high IF journal without possibility to publish open access (due to high charges)?
01 January 1970 460 5 View
Good day, dear collegues. When do you read different types of biomedical scientific articles (i.e. original articles, reviews, letter to editor etc.) at which moments you pay attention ? Do you...
01 January 1970 1,247 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View