This is a plasmid Miniprep, and the 230nm reading is very low. I measured a different Miniprep and the results were perfectly "normal" (higher 230nm reading), so I know it's not an issue with the buffer I used to make the blank reading.
- did the spectra look smooth and nice below 260 nm?
(if the blank has too much absorbence the spectra becomes noisy and meaningless)
Hi Daniel
Minipreps of plasmids are often not as clean as you would think. A common mistake is to use more cells than recommended in an effort to maximize the amount of DNA recovered. This often leads to insufficient lysis resulting in the carryover of unwanted material like carbohydrates and cell membranes.
Regarding the 230 nm measurement I am not really sure what is going on. Usually, impurities like EDTA or carbohydrates will absorb at 230 giving a higher 230 value. If you meant to write that you see a lower 260/230 ratio (which is the interesting) parameter here, then I believe it is caused by some impurity in your miniprep.
Hope this helped
Jesper
Do not completly depend on Nano drop results.Check plasmid concentraion on agarose gel also, sometimes only Nano drop results can not be reliable.
all the best
Qubit fluorometer enables very accurate and specific DNA quantification. With Nanodrop analyze the entire spectrum- it will tell the complete story not only about yield but purity and to some extent - nature of contaminants
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