For small volumes of solutions in the range of 5-30 μL, pipette mixing is generally considered a better option than vortexing. Here's why:

While pipette mixing is generally preferred for small volumes, it's important to consider the specific requirements of your experiment or application. For example, if your solution contains particles that require more vigorous mixing, vortexing may be necessary. Additionally, some specialized applications may recommend alternative mixing methods based on the specific characteristics of the solution or the desired outcome.

In summary, pipette mixing is often the preferred choice for small volumes of solutions (5-30 μL) due to its precision, sample retention, reduced shear stress, and contamination prevention.

Thank you Michelle for taking time and writing sharing your opinion in great details.

It helped a lot.