I wanted to know the principle behind working of paxgene blood tubes. How do they keep RNA safe from degradation? And also whether it is possible to extract DNA from blood stored in paxgene RNA tubes.
(1) Charging period corresponded to the period where electrons transferred from the fixed electrode to the flow electrode at an applied voltage of −0.45 V (vs. Ag/AgCl), and (2) the discharging...
04 June 2024 4,686 0 View
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28 June 2023 5,329 0 View
I am doing an experiment where I see in IF that the localization of the protein is such that it gets dispersed all over the cell from just being perinuclear. How would I perform the analysis in...
22 June 2023 2,524 0 View
I feel small vulumes of solutions are mixed best when pipette mixed instead of vortexi ng.
05 May 2023 4,210 2 View
In an RF sputtering system, how do I change the substrate bias voltage?
07 April 2023 9,993 1 View
how to seprate mixture of Acetonitrile 20% + IPA 80% ?
25 July 2022 9,999 2 View
For most experiments, people use Streptavidin Sepharose beads (affinity of >= 300nm free biotin) (GE Healthcare). However, we would like to use Streptavidin Agarose (affinity of >=120nm free...
04 July 2021 4,904 1 View
Which solvent blank (Media + MTT + Solubilizer ?) should I subtract from the sample to get the corrected absorbance? Does negative cytotoxicity make sense?
14 April 2021 6,823 4 View
What is the role of isopropanol in cryopreservation of cells
13 April 2021 655 1 View
I am trying to measure ROS production in HT22 cell line post 24 hours of treatment in 24 well plate. The two protocols which I have found: 1. post 24 hour of treatment to incubate 5uM of DCFDA by...
20 October 2020 4,433 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View