I am doing an experiment where I see in IF that the localization of the protein is such that it gets dispersed all over the cell from just being perinuclear.
How would I perform the analysis in ImageJ for putting up a quantification?
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I want to know more about diamond ore deposits in world.
11 August 2024 2,167 1 View
We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
10 August 2024 6,678 14 View
If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
10 August 2024 8,198 5 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
What are a “Farmers Producer Organization” (FPO) and its essential features?
10 August 2024 477 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
08 August 2024 4,831 4 View
How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
08 August 2024 8,162 0 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I have images of film formed on the bottom of a 50mL beaker, and I have been trying to figure out the surface area. I could easily use features on imageJ to do that, but I want to know which area...
23 July 2024 4,461 1 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...
11 July 2024 2,257 1 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...
11 July 2024 3,557 3 View
I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...
11 July 2024 1,464 7 View