Currently I am using the protocol of O’Toole and Kolter (O'Toole and Kolter, 1998) for Biofilm formation assay. Now, I would like to apply a recombinant protein to see if there is any inhibitory effect on biofilm formation. I have gone through couple of methods but not convinced yet. I will be grateful if someone help me get a good method for biofilm inhibition study.
Yes, this is O’Toole and Kolter (O'Toole and Kolter, 1998) method
Hi!
I was working on growing biofilms rather than inhibiting them, but I found MBEC assay a neat tool (http://www.innovotech.ca/products_mbec.php). It's a lid with plastic pegs coated with a variety of materials that fits over a 96-well plate. Biofilms grow on pegs and it's easy to maintain.
There's also a list of quite a few publications using the plates on their website, you should be able to find a protocol that would suit your needs, if you want to give it a try.
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