Is there an effective way to detect endogenous contamination (bacteria and fungi) in plant tissue culture? My test method is to inoculate some of the target plants into the Luria-Bertani broth and Potato Dextrose Agar medium to check for bacterial and fungal contamination. However, in some cases, This test method does not work correctly and clearly. Personally, I suspect that there is a limit to the cultivation of various bacteria because Luria-Bertani broth is more optimized for some bacteria. Also, it is inefficient to test twice. Some researchers use a method of testing bacteria and fungal contamination at once using Tryptic Soy Broth medium, is this more efficient and accurate? I ask for the opinions of those who study plant tissue culture.
Dear Andew Bae as you´ve suggested, some microorganisms are difficult to culture, since they need especific CMs to growth. Thus, what culture media you should use to detect endophytics? It depends both of plant species and the occuring microorganisms. In my case, I mostly use M523, which's suitable for most of general situations. However, you must to consider that some microorganisms are difficult to isolate, in this case likely the most suitable detection method is using molecular techniques.
Here you can find the recipe of M523
https://www.duchefa-biochemie.com/product/category/id/21/name/phytopathologica-microbiology
As you can see, it's specific for bacteria indexing. However, it's a nutritive medium where both fungi and yeasts can also growth
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