More importantly, I have noted that this resin is used as a reverse phase sorbent in chromatography. Does it work much the same way as Daion? Is it a typical reverse phase tool?
Do you mean to write Amberlite, manufactured by Rohm Haas? Dow, Rohm Haas, Mitsubishi, and some others offer ion exchangers and adsorbents where the bonded phases of a particular chemistry are some what similar but the backbone polymers are different and therefore have different alpha values for the same bonded functionality. I'm not sure which Amberlite resin you are comparing to Diaion but if it's adsorbent modality then both bonded phases and backbone will differ. I'm not sure if the Amberlite resin you are referring to is available in 5 micron particle size which would make a difference with Diaion if you want to do high pressure HPLC. But the Amberlite can be used for open column applications and for reverse phase purposes.
Do you mean XAD-8 and XAD-16? I've used XAD-16 as a reverse-phase resin for primary extraction from microbial or fungal fermentation. Wash the resin well with water, methanol, and water again before use. I would stir the resin with the filtered fermentation, wash with water, then wash with 25, 50, 75, and 100% methanol.
- Badges
- Science method